婴儿早期艰难梭菌感染可导致肠道损伤

Stem cells cells Goblet cells Tuft cells Absorptive Mock 5 pM 25 pM 200 pM 6.4 nM Industrialized rialized Transitional Not Sampled H Fig. 5. Human infants are susceptible to early-life C. difficile–mediated damage. RNA was harvested from primary human infant IECs isolated from colonic biopsies from C. difficile–negative (infant A) and C. difficile–positive (infant C) patients for RT-qPCR assessment of stem cell (A) and differentiated cell (B) markers. n = 1 infant with three technical replicates per group. (C) Two-dimensional primary infant IEC cultures were treated with rTcdB (shades of red) or vehicle (mock). Data are represented as lactate dehydrogenase release normalized to mock (0% death) and Triton X-100 (100% death) treatment groups. n = 3 passages per group. …