复制后人类染色体姐妹染色单体构象不对称性研究

Flavia Corsi, Thomas L. Steinacker†, Sofia Kolesnikova†, Zsuzsanna Takács, Paul Batty, Michael Mitter, Daniel W. Gerlich*, Anton Goloborodko* INTRODUCTION: DNA replication creates two sister chromatids that must acquire specific three-dimensional conformations to support genome function and faithful segregation. Cohesin complexes help organize replicated chromosomes in two ways: One pool of cohesins extrude chromatin loops within each chromatid, whereas another pool of cohesive cohesins tether the two sisters together. Although sister chromatids are identical in DNA sequence, the replication process is intrinsically asymmetric. Each chromatid inherits a different parental strand, whereas the new strands are synthesized by distinct leading- and lagging-strand machineries at the replication fork. …