亚纳升胶囊中的单细胞与活细胞培养多步骤基因组学

Full article and list of author affiliations: https://doi.org/10.1126/ science.ady7209 Ignas Mazelis, Haoxiang Sun, Arpita Kulkarni, Theresa L. Torre, Allon M. Klein* INTRODUCTION: Single-cell genomic technologies have transformed our ability to measure molecular states across thousands to millions of cells in parallel. Yet these approaches are inherently destructive, making it difficult to connect live-cell behaviors with subsequent molecular readouts. By contrast, assays on living cells can reveal dynamic behaviors and causal mechanisms, but they are limited in their molecular breadth. As a result, many dynamic properties of cells, such as the persistence of epigenetic memory or the response of cells to ligands, remain hard to analyze systematically at scale. …