RNA引导的桥接重组酶实现人类细胞可编程基因组编辑

Oana Pelea1, András Tálas2†, Javier Fernández Carrera1†, Nicolas Mathis2†, Lilly van de Venn3†, Charles D. Yeh3†, Péter I. Kulcsár2†, Kim F. Marquart2, Yanik Weber2, Saskia E. Gerecke1, Isabelle F. Harvey-Seutcheu1, Dominic Mailänder3, Moritz M. Pfleiderer1, Christelle Chanez1, Jacob E. Corn3, Gerald Schwank2, Martin Jinek1* Site-specific insertion of gene-sized DNA fragments remains an unmet need in the field of genome editing. IS110-family serine recombinases have recently been shown to mediate programmable DNA recombination in bacteria by using a bispecific RNA guide (bridge RNA) that simultaneously recognizes target and donor sites. In this work, we have shown that the bridge recombinase ISCro4 is highly active in human cells and provided structural insights into its enhanced activity. …