将1/2组框架扩展至更多小鼠品系与人类转录组

Fig. 4. Extension of the group 1/group 2 framework to additional mouse lines and human transcriptomes. (A) Pairwise Pearson correlations of log2 FC (compare with Fig. 1C) were recomputed after projecting three additional lines, Chd8-S62X, Ank2, and Shank2, onto the classifier. Chd8-S62X: group 1; Ank2, Shank2: group 2. (B) Human transcriptome grouping. Hierarchical clustering of log2 fold change profiles from BA9 RNA-seq (40 autistic individuals, ages 2 to 67 years) yields hgroup 1 (n = 20) and hgroup 2 (n = 20). Annotation bars show sex, age, brain weight, and seizure history. (C) Biological signatures of human subgroups. GSEA (GO, BP/CC) reveals largely opposite changes in biological functions, including immune, extracellular matrix/ECM, and synapse. …