脾脏微环境在稳态下储存部分活化的血小板池

We concluded that platelets were partially activated in the spleen, but this did not lead to permanent adhesion; rather, it allowed for recirculation. We did not observe overt differences in the phenotype, activation, adhesion, and clot formation ability in steady state of peripheral blood platelets from splenectomized mice (fig. S5, B to F, and fig. S6). Moreover, we observed normalization of platelet turnover and lifespan in the chronic phase after splenectomy (fig. S6, A and B). Our pulse-labeling approach allowed for specific analysis of clearance of aged platelets (>72 hours old), which revealed similar turnover of this cohort in splenectomized compared with sham mice, excluding a major role of the spleen in clearance of this population in homeostasis (fig. …