营养扰动重塑肝细胞沿门静脉-中央静脉轴的亚区异质性

Fig. 4. Nutritional perturbations remodeled subzonal hepatocyte heterogeneity across the PV–CV axis. (A to C) Representative confocal images of liver acini from control- fed (A), overnight- fasted (B), and 4- week Western diet (WD)- fed (C) mice labeled for cell boundaries and organelles. Insets show periportal (PV) and pericentral (CV) regions. Scale bars, 100 μm; inset, 5 μm. (D) Spatial heatmaps of selected organelle features across the normalized PV–CV axis for control (n = 4 mice), fasting (n = 3 mice), and WD (n = 3 mice) groups. Colors represent z- scored feature values within each condition. (E) PCA of hepatocytes (~10,500 cells total) from control, fasting, and WD groups. …