染色质在转录过程中缓冲扭转应力

Jin Qian1,2, Lucyna Lubkowska3, Shuming Zhang1,2, Chuang Tan1,2, Yifeng Hong4, Xiaomeng Jia1,2, Robert M. Fulbright1, James T. Inman1,2, Taryn M. Kay5, Joshua Jeong6, Glenn Hauk6, Deanna Gotte7, James M. Berger6, Mikhail Kashlev3, Michelle D. Wang1,2* During eukaryotic transcription, RNA polymerase II (Pol II) must overcome nucleosome obstacles and, because of DNA’s helical structure, must also rotate relative to DNA, which generates torsional stress. However, there is limited understanding of how Pol II transcribes through nucleosomes while supercoiling DNA. In this work, we determined that Pol II generates a torque of 9 piconewton-nanometers (pN·nm) alone and 13 pN·nm with transcription factor IIS (TFIIS), making it a powerful rotary motor. …