一种用于哺乳动物细胞转录组存储的基因编码装置

Yu-Kai Chao1,2, Michelle Wu1, Qiyu Gong1, Fei Chen1,3* Understanding how cells make decisions over time requires the ability to link past molecular states to future phenotypic outcomes. We present TimeVault, a genetically encoded system that records and stores transcriptomes within living mammalian cells for future readout. TimeVault leverages engineered vault particles that capture messenger RNA through polyadenosine [poly (A)]–binding protein. We demonstrate that the transcriptome stored by TimeVaults is stable in living cells for more than 7 days. TimeVault enables high-fidelity transcriptome-wide recording with minimal cellular perturbation, capturing transient stress responses and revealing gene expression changes underlying drug-naïve persister states in lung cancer cells that evade epidermal growth factor receptor (EGFR) inhibition. …