高分辨率冷冻电镜揭示管状鞭毛茸毛的N-和O-聚糖结构

Structural N- and O- glycans revealed by high- resolution cryo- EM analysis of tubular mastigonemes Full article and list of author affiliations: https://doi.org/10.1126/ science.aef4958 Junhao Huang (黄隽豪)†*, Hui Tao (陶慧)†, Sheng Chen (陈晟)†, Yahua Cui (崔亚华), Yiran Xu (徐艺然), Chuangye Yan (闫创业)*, Nieng Yan (颜宁)* INTRODUCTION: Glycans, the largest biomass on Earth, function in a broad spectrum of cellular activities in addition to their central roles in metabolism. Yet glycan structural biology remains severely hampered by chemical complexity, conformational flexibility, and nontemplated synthesis. The CryoSeek strategy, which represents a structure- first paradigm for discovery, has yielded dozens of high- order structures of glycans in the form of glycofibrils. However, the ~3.0- Å resolution of these glycans precludes reliable sugar assignment. …